Vitamin D, 25-Hydroxy and 1,25-Dihydroxy Panel
A full vitamin D workup that pairs your storage status with the active hormone the kidney makes.
Consider this test if:
- Working up unexplained hypercalcemia or abnormal calcium and parathyroid results
- Chronic kidney disease, where activation of vitamin D is impaired
- Suspected hyperparathyroidism (primary, secondary, or tertiary)
- Investigating rickets or a granulomatous condition like sarcoidosis
- Wanting both your vitamin D stores and the active hormone in one draw
- HSA/FSA eligible
- Results delivered to your dashboard · Reviewed by a real clinician
- Drawn at a CLIA/CAP-accredited lab near you ·
Pre-test considerations
No fasting required. If you take vitamin D, biotin, or other supplements, note the dose and timing, since recent supplementation raises 25-hydroxy levels; keep your routine consistent if you plan to compare results over time.
Video consult with your Care Team
A 1:1 call to discuss your health.
What members say on Trustpilot
What this test is for
This profile measures both major circulating forms of vitamin D: 25-hydroxyvitamin D, the storage form that reflects your overall vitamin D status from sun, diet, and supplements, and 1,25-dihydroxyvitamin D (calcitriol), the active hormone the kidney produces to regulate calcium. Each is broken out into its D2 and D3 components, which helps distinguish dietary or supplemental intake from what your body makes. The 25-hydroxy result is the right marker for the everyday question of whether your level is adequate, while the 1,25-dihydroxy result is used for specific calcium, kidney, and parathyroid problems: vitamin D status in renal failure, the differential diagnosis of primary, secondary, and tertiary hyperparathyroidism, the workup of hypercalcemia, and inherited rickets.
Most people checking routine vitamin D status need only the 25-hydroxy test; this complete profile is for cases where the active form genuinely adds information, such as unexplained high calcium or known kidney or parathyroid disease.
Biomarkers tested
Includes 6 biomarkers
- Specimen
- Serum or plasma
- Measures
- Mass concentration
- Specimen
- Serum or plasma
- Measures
- Mass concentration
- Specimen
- Serum or plasma
- Measures
- Mass concentration
- Specimen
- Serum or plasma
- Measures
- Mass concentration
- Specimen
- Serum or plasma
- Measures
- Mass concentration
- Specimen
- Serum or plasma
- Measures
- Mass concentration
What you'll learn
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Insights that connect the dots. A clinician reads your whole picture, not one result in isolation, connecting family history, past results, and lifestyle into clear next steps.
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Your personalized action plan. Nutrition, supplements, and training in one clinician-reviewed protocol, updated as your results change.
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Track your trends. Upload results from any lab and see each biomarker trend over time, so you know what is working.
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Never miss a recheck. Mito tells you what is worth retesting and when, then reminds you so nothing slips.
What to expect
- 1 Book instantly
Click, book, done. Choose a convenient lab location near you. Transparent, up-front pricing.
- 2 Quick lab visit
Testing to fit your busy schedule, usually 15 minutes or less. Walk-in and appointments available.
- 3 Fast, dashboard-delivered results
Your results post straight to your dashboard as soon as the lab completes them.
- 4 Expert guidance
Included with Mito membership. A clinician reviews your results and your personalized action plan follows, with clear next steps.
Everything your health needs,
in one membership
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Every test at our cost
Members pay our cost on every test, with lab fees passed straight through. The full receipt is itemized, never padded.
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Continuous tracking, all in one place
Upload past labs and watch your trends over time. Every marker and visit lives in one longitudinal record, so all your care stays together.
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Year-round clinician support
Text anytime and get clinician-reviewed answers. When you want to go deeper, 1:1 consultations are available at affordable rates.
All for $9/month
Order any test or consult without joining. For $9/month, members unlock member prices, trend tracking, and year-round clinician guidance.
Mito Membership
$9 /mo
cancel anytime
Without membership
$0
pay as you go
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On-demand clinician consults when you need guidance
Personalized action plans across supplements, exercise, nutrition, and sleep
AI health coaching to help you act on your results
Year-round medical support, with in-chat clinician escalation
All your health records in one personal vault, with trends and biological age tracking
Early access to new diagnostics and product releases
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Frequently asked questions
View all FAQsHow does pricing work?
Every test shows the member price next to the standard non-member price, so you can see what membership saves you. The member price is our cost, covering the lab and what it takes to run the service. It is never a profit on the test itself. Mito makes its money on the $9 membership, not on marking up your tests. Membership is $9 per month, and any applicable lab order or draw fee is itemized before you pay.
Where do I get tested?
Choose an available partner lab at checkout. If your cart contains multiple blood tests, Mito helps consolidate the eligible tests at one lab when possible. At-home kits and scans follow the collection or appointment process shown on their product page.
Is this eligible for HSA/FSA?
Yes. Eligible tests can be paid for with an HSA or FSA card at checkout.
When will I get my results?
Your results appear in your Mito dashboard after the laboratory completes processing. A clinician then reviews them before your analysis and personalized action plan are finalized. Timing varies by test, and specialty assays and at-home kits may take longer.
Do I need a physician's order?
No. Mito provides the laboratory order when one is required, so you do not need to arrange a separate physician visit before purchasing.
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