Vitamin D, 25-Hydroxy and 1,25-Dihydroxy Panel
A full vitamin D workup that pairs your storage status with the active hormone the kidney makes.
Consider this test if:
- Working up unexplained hypercalcemia or abnormal calcium and parathyroid results
- Chronic kidney disease, where activation of vitamin D is impaired
- Suspected hyperparathyroidism (primary, secondary, or tertiary)
- Investigating rickets or a granulomatous condition like sarcoidosis
- Wanting both your vitamin D stores and the active hormone in one draw
- HSA/FSA eligible
- Results delivered to your dashboard · Reviewed by a real clinician
- Drawn at a CLIA/CAP-accredited lab near you ·
Pre-test considerations
No fasting required. If you take vitamin D, biotin, or other supplements, note the dose and timing, since recent supplementation raises 25-hydroxy levels; keep your routine consistent if you plan to compare results over time.
Video consult with your Care Team
A 1:1 call to discuss your health.
What members say on Trustpilot
What this test is for
This profile measures both major circulating forms of vitamin D: 25-hydroxyvitamin D, the storage form that reflects your overall vitamin D status from sun, diet, and supplements, and 1,25-dihydroxyvitamin D (calcitriol), the active hormone the kidney produces to regulate calcium. Each is broken out into its D2 and D3 components, which helps distinguish dietary or supplemental intake from what your body makes. The 25-hydroxy result is the right marker for the everyday question of whether your level is adequate, while the 1,25-dihydroxy result is used for specific calcium, kidney, and parathyroid problems: vitamin D status in renal failure, the differential diagnosis of primary, secondary, and tertiary hyperparathyroidism, the workup of hypercalcemia, and inherited rickets.
Most people checking routine vitamin D status need only the 25-hydroxy test; this complete profile is for cases where the active form genuinely adds information, such as unexplained high calcium or known kidney or parathyroid disease.
Biomarkers tested
Includes 6 biomarkers
- Specimen
- Serum or plasma
- Measures
- Mass concentration
- Specimen
- Serum or plasma
- Measures
- Mass concentration
- Specimen
- Serum or plasma
- Measures
- Mass concentration
- Specimen
- Serum or plasma
- Measures
- Mass concentration
- Specimen
- Serum or plasma
- Measures
- Mass concentration
- Specimen
- Serum or plasma
- Measures
- Mass concentration
What to expect
- 1 Book instantly
Click, book, done. Choose a convenient lab location near you. Transparent, up-front pricing.
- 2 Quick lab visit
Testing to fit your busy schedule, usually 15 minutes or less. Walk-in and appointments available.
- 3 Fast, dashboard-delivered results
Your results post straight to your dashboard as soon as the lab completes them.
- 4 Expert guidance
Included with Mito membership. A clinician reviews your results and your personalized action plan follows, with clear next steps.
Everything your health needs,
in one membership
-
Every test at our cost
Members pay our cost on every test, with lab fees passed straight through. The full receipt is itemized, never padded.
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Continuous tracking, all in one place
Upload past labs and watch your trends over time. Every marker and visit lives in one longitudinal record, so all your care stays together.
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Year-round clinician support
Text anytime and get clinician-reviewed answers. When you want to go deeper, 1:1 consultations are available at affordable rates.
All for $9/month
Order any test or consult without joining. For $9/month, members unlock member prices, trend tracking, and year-round clinician guidance.
Mito Membership
$9 /mo
cancel anytime
Without membership
$0
pay as you go
At-cost pricing on labs, scans, and more
Priority turnaround on your results
On-demand clinician consults when you need guidance
Personalized action plans across supplements, exercise, nutrition, and sleep
AI health coaching to help you act on your results
Year-round medical support, with in-chat clinician escalation
All your health records in one personal vault, with trends and biological age tracking
Early access to new diagnostics and product releases
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Frequently asked questions
View all FAQsHow does pricing work?
Every test shows the member price next to the standard non-member price, so you can see what membership saves you. The member price is our cost — covering the lab and what it takes to run the service — never a profit on the test itself; Mito makes its money on the $9 membership, not on marking up your tests. Membership is $9/mo, and you still pay the lab’s order fee. Prices are itemized before you pay, with no hidden fees.
Where do I get tested?
Choose a partner lab (Quest, Labcorp, or BioReference) at checkout. If your cart spans multiple tests, we consolidate the whole order onto a single lab so you only make one visit.
Is this eligible for HSA/FSA?
Yes. This test is HSA/FSA eligible, and you can pay with your HSA/FSA card at checkout.
When will I get my results?
Your results post to your dashboard once your lab completes them, then a clinician reviews them and your full analysis and personalized action plan (with clear next steps) follow. Turnaround varies by test: specialty assays and at-home kits take longer, and each test shows its expected turnaround before you buy.
Do I need a doctor’s order?
No. Mito provides the lab order for you, so you can book and get tested without a separate doctor visit.
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