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HIV-1/2 Antigen and Antibodies, Fourth Generation, with Reflexes
Blood test

HIV-1/2 Antigen and Antibodies, Fourth Generation, with Reflexes

$24.99 Member price $34.99 Non-member
Excellent 4.8 out of 5

The standard fourth-generation screen for HIV-1 and HIV-2, able to catch infection earlier than antibody-only tests.

Consider this test if:

  • Starting or restarting PrEP and confirming your status first
  • A possible exposure through sex, needle sharing, or occupational contact
  • Routine screening as recommended for anyone aged 15 to 65, or during pregnancy
  • HSA/FSA eligible
  • Typical results in 1-2 days · Reviewed by a real clinician
  • Drawn at a CLIA/CAP-accredited lab near you ·
Reflex testing

If your initial screen comes back positive, follow-up testing runs automatically to confirm the result and distinguish HIV-1 from HIV-2, including RNA testing when needed to catch acute infection before antibodies form. These reflex steps are included at no additional cost.

Pre-test considerations

No fasting required and no special timing during the day. Timing relative to exposure matters: the p24 antigen becomes detectable roughly two to four weeks after infection, so a very recent exposure can still test negative and may need a repeat test after the window closes.

Video consult with your Care Team

A 1:1 call to discuss your health.

$39.00 $99.00 Non-member

What members say on Trustpilot

  • “Mito gives me 3x the tests for less money” · Page
  • “15/10 experience - I felt actually taken care of” · Reena Sudan
  • “Deeper insights than a typical annual physical” · Nicki H
  • “Loved how specific and actionable the advice was” · Maya Hickey
  • “Stunned at how thorough both the testing and analysis was” · Mark
  • “Tired of arguing with my PCP. Mito got me the answers I was looking for.” · Josh
  • “So easy, it feels like a deluxe experience for your health” · Menetreto
  • “Excellent responsive customer service (a real rarity these days!)” · SS

What this test is for

This is a fourth-generation HIV screen that detects HIV-1 and HIV-2 antibodies plus the HIV-1 p24 antigen in a single test. Because p24 antigen appears before antibodies develop, it can identify acute infection during the early window when antibody-only tests still read negative. It is the CDC-recommended starting point for HIV diagnosis in adults, pregnant women, and children two years and older, with over 99% sensitivity and specificity. It is the right test for routine screening, for confirming status before starting PrEP, and for checking after a possible exposure once enough time has passed for the marker to turn positive.

This catalog item is the standalone fourth-generation screen with the full reflex algorithm. The same antigen/antibody assay also appears bundled inside our broader STI serology panel alongside syphilis, hepatitis B, hepatitis C, and herpes.

Specimen
Serum or plasma
Method
Immunoassay
Measures
Presence / threshold
Mito dashboard showing biological age, health categories, biomarkers, and health trends

What you'll learn

  • Insights that connect the dots. A clinician reads your whole picture, not one result in isolation, connecting family history, past results, and lifestyle into clear next steps.

  • Your personalized action plan. Nutrition, supplements, and training in one clinician-reviewed protocol, updated as your results change.

  • Track your trends. Upload results from any lab and see each biomarker trend over time, so you know what is working.

  • Never miss a recheck. Mito tells you what is worth retesting and when, then reminds you so nothing slips.

What to expect

  1. 1 Book instantly

    Click, book, done. Choose a convenient lab location near you. Transparent, up-front pricing.

  2. 2 Quick lab visit

    Testing to fit your busy schedule, usually 15 minutes or less. Walk-in and appointments available.

  3. 3 Typical results in 1-2 days

    Your results post straight to your dashboard as soon as the lab completes them.

  4. 4 Expert guidance

    Included with Mito membership. A clinician reviews your results and your personalized action plan follows, with clear next steps.

Find a lab near you

Every draw site is a CLIA/CAP-accredited partner lab. Enter your ZIP to see the nearest locations.

    Everything your health needs,
    in one membership

    • A Mito member relaxing at home, checking their results on their phone

      Every test at our cost

      Members pay our cost on every test, with lab fees passed straight through. The full receipt is itemized, never padded.

    • Mito dashboard on a tablet showing biomarker results and trends

      Continuous tracking, all in one place

      Upload past labs and watch your trends over time. Every marker and visit lives in one longitudinal record, so all your care stays together.

    • A Mito clinician checking in with a member over text messaging

      Year-round clinician support

      Text anytime and get clinician-reviewed answers. When you want to go deeper, 1:1 consultations are available at affordable rates.

    All for $9/month

    Order any test or consult without joining. For $9/month, members unlock member prices, trend tracking, and year-round clinician guidance.

    Mito Membership

    $9 /mo

    cancel anytime

    Without membership

    $0

    pay as you go

    At-cost pricing on labs, scans, and more

    Standard pricing

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    On-demand clinician consults when you need guidance

    $39 per 30 min
    $99 per 30 min

    Personalized action plans across supplements, exercise, nutrition, and sleep

    AI health coaching to help you act on your results

    Year-round medical support, with in-chat clinician escalation

    All your health records in one personal vault, with trends and biological age tracking

    Early access to new diagnostics and product releases

    Frequently asked questions

    View all FAQs
    How does pricing work?

    Every test shows the member price next to the standard non-member price, so you can see what membership saves you. The member price is our cost, covering the lab and what it takes to run the service. It is never a profit on the test itself. Mito makes its money on the $9 membership, not on marking up your tests. Membership is $9 per month, and any applicable lab order or draw fee is itemized before you pay.

    Where do I get tested?

    Choose an available partner lab at checkout. If your cart contains multiple blood tests, Mito helps consolidate the eligible tests at one lab when possible. At-home kits and scans follow the collection or appointment process shown on their product page.

    Is this eligible for HSA/FSA?

    Yes. Eligible tests can be paid for with an HSA or FSA card at checkout.

    When will I get my results?

    Your results appear in your Mito dashboard after the laboratory completes processing. A clinician then reviews them before your analysis and personalized action plan are finalized. Timing varies by test, and specialty assays and at-home kits may take longer.

    Do I need a physician's order?

    No. Mito provides the laboratory order when one is required, so you do not need to arrange a separate physician visit before purchasing.

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